Elucidation of the Chemical Structure of Anti-Microbe Active Material That the Leaf Plane Bacteria Produces

For further information, see Hiradate et al. Phytochemistry, 61, 693-698 (2002)
and Yoshida et al., JARQ, 36, 89-95 (2002)., 2003-12-01

Interaction exists between two or more living things, such as plant, microbe, and insect. Fundamental research on the chemical substance, complicated chain systems, and action mechanisms involved in such interaction are now advanced.

Biota on Leaf Surface Control Microbes

What produces and secretes anti-microbe activity substance and controls other microbes is the fine biota which exists in the surface of a vegetable leaf. It is known as the bacteria B. amyloliquefaciens which inhabits the surface of the leaf of a mulberry and suppresses the development of symptoms of mulberry anthracnose. Such action, in effect, has positive effects on the growth of mulberry.

Chemical Structure of Antimicrobial Materials

However, since the chemical structure of the active anti-microbe material has not been specified, the action mechanism has not been fully understood. The B. amyloliquefaciens active material, which is amyloliquefaciens, was isolated in this study and its chemical structure determined.

B. amyloliquefaciens RC-2 was cultivated massively in the liquid medium, and the growth inhibition activity for Colletotrichum dematium was made as an index. Anti-microbe active material was refined from the culture filtrate, and seven kinds of chemical compounds were finally isolated.

As a result of analyzing the chemical structure of these anti-microbe active materials by various spectral analyses, the cyclic peptide chemical compound called Iturin was identified.

Within the specified anti-microbe active material, it became clear that the chemical compound Iturin A-8 was a new material.

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