High-Precision and High-Efficient QTL Analysis for Iron Resistance of Rice and Fusarium Disease Resistance of Barley

For further information, see Hori, K.; Shimizu, T.; Satou, K.; Takeda, K.; Kawasaki, S. Theor. Appl. Genet. (Accepted)., 2003-11-01

High Efficiency Genome Scanning

This study developed a time- and cost-saving QTL precision analysis for iron repletion resistance of rice plant with complicated genotypic composition, as well as for rust resistance of barley, through the use of High Efficiency Genome Scanning for adequate recombination inbred lines.

QTL Analysis for Chacteristics Involving Multiple Genes

The QTL analysis is a powerful technique for analyzing complicated characteristics involving multiple genes. However, the precision of the analysis may not be sufficient when a large number of genetic markers, such as 100-200 individuals, are involved; also, considerable time and costs are needed. Hence, it is necessary to develop a high-precision QTL analysis which is both time and cost effective, and involves high-efficient genome scanning to enable the manufacture of precise genome map.

Leaf Discoloration of Rice Growing in Laterite Soils

In the tropical zone, red soil which mainly contains iron oxide called the laterite is widely distributed, and when such land is made into paddy field and begins to dissolve in water, non-resistant rice plants suffer from leaf discoloration, becoming reddish brown, causing growth inhibition. The rice type Indica, which is mainly grown in the tropical zone, has weak resistance to iron, but the Japonica rice type grown in the temperate zone has comparatively higher resistance to iron.

Mapping of Rust Resistance

This study was able to produce high-density map that consists of 1300 markers within half a year, using recombination inbred lines between high-resistant Ginbouzu (Japonica) and low-resistant Kasalath (Indica).

The high-density map was produced by using HEGS/AFLP which was a recombination of inbred lines H.E.S.4 which is sensitive and Russia No. 6 which is rust resistant, in cooperation with the Okayama University resources biology laboratory. Simultaneously, the sitting position of three almost equivalent titers as a result of the QTL analysis of resistance by using the limits spike method was clearly confirmed. The strong interaction existing between this gene and the other genes was also clarified.

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