Development of an Elisa Kit to Detect Antibodies against FMD Virus

News source: National Veterinary Research Institute, Taiwan ROC
For further information: hc@mail.nvri.gov.tw, 2003-06-01

Quick Elisa Test for FMD

The O/TAW/97 foot-and-mouth disease virus (FMDV) was propagated in BHK-21 cells. Inactivated viral antigen was prepared by BEI inactivation, PEG concentration, and ultracentrifugation. Two approaches were applied to the test. One was coated with FMDV directly on the 96 wells immunoplate. The other was coated with rabbit anti-FMDV antibodies on the 96 wells immunoplate, and then the FMDV antigens were combined with rabbit antiserum in the following steps. In order to set up the test procedure, the optimal concentration of FMDV antigen, detection antibody, and conjugate were determined in advance. Then, 320 negative swine sera were applied to the test for determining the cutoff value, and at the same time, 475 swine sera were detected by the newly developed kit and ELISA-antibody detection kit purchased from Pirbright FMD WRL in UK.

Good Results from Tests

Results showed that the kappa value was 0.93 between the new kit and the Pirbright kit. Based on the kappa value, the identity of the new kit and the Pirbright kit was very high. Therefore, the development of ELISA antibody detection kit based on O/TAW/97 antigen was completed.

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