Determination of Chloramphenicol in Feed Use of High-Performance Liquid Chromatographic-Mass Spectrometry

News source: Branch Institute of Animal Drugs Inspection, National Veterinary Research Institute, Miaoli, Taiwan ROC
For further information: Teresa_kuo1231@yahoo.com.tw, 2003-06-01

Detecting the Antibiotic Chloramphenicol

The use of performance liquid chromatographic-mass spectrometry (HPLC-MS) for the determination of chloramphenicol in feed was developed in the laboratory. Feed was extracted with acetonitrile and purified with Sep-Pak Alumina N cartridge. The X-Terra MS C18 Column and 20% acetonitrile were used as mobile phase to perform the levels of 0.1, 0.5, 1.0, and 5.0 ppm of chloramphenicol in the feed. The average recovery rate was 81.6-91.8%. This result showed that HPLC-MS is useful in detecting specific molecular weight of chloramphenicol.

Used to Check Veterinary Drugs

In 2002, a total of 3,414 batches of commercial veterinary chemical drugs were sampled from the market and checked by official method. Results revealed that 25 sessions of visits and inspections for good manufacturing practices in veterinary chemical drug factories can be accomplished.

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