Development of a Bovine Coronavirus Diarrhea Inactivated Vaccine

News source: National Veterinary Research Institute, Taiwan ROC
For further information: bioprod@mail.nvri.gov.tw, 2003-08-01

New Vaccine for Bovine Coronavirus

Bovine coronavirus (BCV) was propagated in HRT-18 cells. Vaccine was produced from the infected virus suspension inactivated with 0.002 M Binary Ethylene Imide (BEI) and incorporated with Montanide oil adjuvant.

Testing of Different Adjuvants for Vaccine

Concerned about the different adjuvants, which may cause various immune responses, aluminum phosphate, MVP emulsigen, and Montanide oil were included in this study. The mean antibody titers tested by the hemagglutination inhibition (HI) were 1:130, 1:144, and 1:176.

Better Antibody Response from Montanide Oil Vaccine

This study showed that vaccine produced by the Montanide oil stimulated better antibody response. The field trial was performed in two cow farms including 50 vaccinated cattle and 6 control cattle. The mean HI titers were 1:26 before vaccination and then increased to 1:264 and 1:240 at the 4th and 8th weeks after the second vaccination, respectively.

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