Callus Differentiation of Cucumber Influence of Light Morphogenesis and Protein Content

News source: Rural Development Administration, Suwon, Korea
For further information, see Korean J. Plant Tissue Culture, v.27(3) pp.213-217., 2003-05-01

Tissue Culture of Cucumber

To investigate cucumber (Cucumis sativus L.) regeneration from embryogenic calli, shoot tips of aseptically-grown cucumber seedlings were used as explants for establishing tissue cultures. Growth and differentiation of callus were studied by using Murashige and Skoog's (MS) medium containing 0.5-2 mg/L 2,4-D.

Induction of Plantlets

Plantlets were induced from the shoot tip culture on the plant growth regulator-free MS medium. Non-embryogenic calli and viscous calli were induced on the medium supplemented with 0.5-2 mg/L 2,4-D, but embryogenic callus was not induced on the same medium.

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