Micropropagation of Cassava Suspension Culture Derived from Nodal Explants

News source: Rural Development Administration, Suwon, Korea
For further information, see Korean J. Plant Tissue Culture, v.27(3) pp.185-189., 2003-05-01

Tissue Culture of Cassava

For the micropropagation, node explants of cassava were cultured in liquid MS medium with various concentrations of cytokinins on a rotary shaker (100 rpm) for 2 weeks.

Better Results from Liquid Than Solid Medium

The adventitious roots and shoots from the explants differentiated more efficiently in liquid medium than in solid medium. However, root formation was not inhibited in the medium with BAP and kinetin at low concentration (>0.05 mh l deci). Meanwhile, in the medium with BAP and zeatin at high level (<0.25 mg l deci), root formation was inhibited by callus forming on the cut end of the cuttings. However, all plantlets grown in liquid medium for more than 2 weeks showed symptoms of hyperhydrocity.

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