Callus and Micro-Crown Bud Formation of Yacon in Vitro from Leaf Explant

News source: Rural Development Administration, Suwon, Korea
For further information, see Korean J. Plant Tissue Culture, v.27(2) pp.101-107., 2003-04-01

Yacon (Earth Apple): An Ancient Crop

Explants of yacon (Polymnia sonchifolia Poepping & Endlicher) were cultured to determine differentiation conditions, and formative callus from the leaf was cultured to investigate regeneration and micro-crown bud formations.

Tissue Culture of Yacon

Basal MS medium was more effective for callus formation than 1/2MS and B-5 medium. Calli formations from the leaf, petiole, and lateral bud were more effective on MS medium supplemented with 1.0, 2.0 mg/L 2,4-D, and 0.2, 0.4 mg/L kinetin or BA than with 1.0, 2.0 mg/L NAA and 0.2, 0.4 mg/L kinetin or BA. Formative callus from the leaf proliferated about 70% on the medium supplemented with 1.0 mg/L BA in subculture for 3-4 months, but not on the medium supplemented with 1.0, 2.0 mg/L kinetin.

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