Transformation of Rice Mediated by Agrobacterium Tumefaciens Effects of Variety and Acetosyringone

News source: Rural Development Administration, Suwon, Korea
For further information, see Korean J. Plant Tissue Culture, v.27(2) pp.95-100., 2003-04-01

GR Gene Introduced into Japonica Rice

The cytosolidc glutathione reductase (GR) gene of Brassica campestris L. was introduced into several Japonica cultivars of rice by Agrobacterium tumefaciens, and a large number of transgenic plants were produced.

Transgenic Plants Compared to Rice Cultivars

Three-week old calli were co-cultivated with A. tumefaciens strain EHA101 carrying the plasmid plGR1. The efficiency of transformation differed from those of rice cultivars. A Japonica cultivar, `Daeribbyeo', appeared to have the highest efficiency (42.5%) of transformation among the four cultivars tested. The addition of acetosyringone (50 muM) during co-cultivation was a key to successful transformation.

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