Effect of Growth Regulators and Antioxidant Mixture on the Anther Floating Cultures of Rice

News source: Rural Development Administration, Suwon, Korea
For further information, see Korean J. Plant Tissue Culture, v.27(2) pp.83-87., 2003-04-01

Callus Induction and Plant Regeneration

To investigate the effect of growth regulators and antioxidant on anther floating cultures of rice, anthers were cultured in liquid media supplemented with different growth regulators, and the effects of antioxidant mixture (Sigma Chemical Co.) on callus induction and plant regeneration were investigated. N6 medium with 1 mg/L 2,4-D and 1 mg/L kinetin was the best for rice anther floating cultures, which showed 48.5% of callus induction and 6.8% of green plant regeneration.

Antioxidant Effective in Reducing Brownish Callus and Improving Plant Regeneration

Callus induction was not affected by antioxidant mixture in liquid medium, and antioxidant mixture (250 mg/L) was effective for the reduction of brownish callus and improvement of plant regeneration.

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