Class I SLG Gene from Chinese Cabbage (Brassica Campestris): Isolation and Characterization

News source: Rural Development Administration, Suwon, Korea
For further information, see Molecules and Cells, v.10(6) pp.678-683., 2003-03-01

Self-Incompatibility in Chinese Cabbage

In brassica species, self-incompatibility in the recognition reaction between self and non-self pollens is determined by two genes, SLG and SRK, at the S locus.

Gene Cloned and Characterized

A genomic DNA fragment containing a complete open reading frame of the SLG gene from Chinese cabbage was cloned and characterized. The genomic clone, named BcSLG2, was found to possess the region that shares a homology of 77% in amino acid identity with the SLG46 gene of Brassica campestris. Northern blot analysis revealed that the BcSLG2 gene expression is restricted to the pistil of Chinese cabbage flower.

Download the PDF. of this document, 23,169 bytes (22.6 KB).