In Planta Visual Monitoring of Green Fluorescent Protein in Transgenic Rice Plants

News source: Rural Development Administration, Suwon, Korea
For further information, see Molecules and Cells, v.10(4) pp.411-414., 2003-03-01

Fluorescent Protein from Jellyfish Used As Reporter

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a widely used reporter that can be directly visualized in the living cells of both animals and plants. A synthetic gene (sgfp) encoding a modified form of the GFP was inserted into expression vector, Act1-sgfp, for the direct expression of GFP which is easily detectable in rice plants.

Synthetic Gene Inserted into Rice Plants

Green fluorescence emitted from GFP could be visualized in calli, dry seeds, roots, and seedlings with green shoots of transgenic rice plants. In this visualization system with a charge-coupled device camera, band-pass filters and a light source indicating the presence of red chlorophyll auto-fluorescence from chloroplasts did not alter the green fluorescence of GFP.

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