Analysis of Opt8-511 Rapd Fragments Closely Linked with Cold Sensitivity at Seedling Stage in Rice (Oryza Sativa L.)

News source: Rural Development Administration, Suwon, Korea
For further information, see Molecules and Cells, 10 (4) pp.382-385., 2003-03-01

Genes for Cold Sensitivity

OPT8-511 was confirmed to be strongly associated with cold sensitivity of rice by random amplified polymorphic DNA (RAPD) analysis for cold tolerance with 94 F-2 population crossed with `Dular' (cold sensitive cultivar) and `Toyohatamochi' (cold resistant cultivar).

Dna Marker Identified

A DNA marker from the RAPD fragment, OPT8-511, has been cloned with genomic DNA from rice cultivar (Dular) and the nucleotide sequence has been determined. The nucleotide sequence revealed that the putative open reading frame was 511 base pairs and contained 169 amino acid residues. It was 79% and 57% identical to the rice cDNA (C26347) in the DataBank at the nucleotide and amino acid sequence levels, respectively.

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