Cell Immobilization of Kluyveroyces Marxianus CBS 6556 Culture on Local Inulin Substrate to Produce Inulinase

News source: Center Research for Biotechnology,
Indonesian Institute of Science
For further information, contact Melliawati, R.; Sufiantini, N.K., Kusnadi, Y., and Prayitno, N.R., Center Research for Biotechnology, Jalan Raya Bogor Km.46, Cibinong 16911, Indonesia, 2003-01-01

Inulin Used As a Carbon Source

INULINASE is one of the enzymes produced by many microorganisms grown on inulin substrate. Inulin is used as a carbon source obtained from plants, especially those belonging to the family Grammineae, such as Elephanthopus spicatus, Crinum asiaticum, Hymenocalis hybrid, H. americana, and Dahlia sp. Immobilization cell of K. marxianus CBS 6556 was prepared to produce inulinase on the medium containing local inulin derived from the tuber of E. spicatus. Natrium alginate was used as an entrapping material of K. marxianus CBS 6556 cells.

Enzme Activity of Immobilization Cells on Mineral Salt Medium

Results showed that immobilization cell with a composition of 10 g Na-alginate and 5 ml yeast suspension was the best concentration. The enzyme activity produced by these immobilization cells on mineral salt medium reached 10.13 unit/ml after 48 hours of incubation at 40oC. Based on this result, local inulin medium was used as a single carbon source in an airlift fermentor. Inulin purified from the tuber of E. spicatus showed the best results. Enzyme activity was 5.48 unit/ml after 48 hours incubation with protein content of 0,16 mg/ml and a specific activity of 31.75 unit/mg proteins. Meanwhile, the inulinase activity produced using free cells reached 23.92 unit/ml with protein contents of 0.28 mg/ml and a specific activity of 85.08 unit/mg proteins.

Use of Free VS. Immobilized Cells

The enzyme activity produced by the free cells was four to five times higher compared with that produced by immobilized cells. However, immobilized cells could be used several times continuously. The use of free and immobilized cells grown on local inulin medium and their enzyme activity was also discussed.

Download the PDF. of this document, 23,149 bytes (22.6 KB).