Rapid Method of Detecting Adulteration of Goats' Milk

News source: Food Industry Research & Development Institute, Taiwan ROC.
See PDF file for e-mail address., 2002-05-01

Adulteration Milk with Cheaper Cows' Milk

An indirect ELISA (enzyme-linked immunosorbent assay) and a sandwich ELISA were successfully developed in this study for the rapid, quantitative detection of cows' milk used to adulterate the more expensive goats' milk.

Polyclonal Antibodies

Polyclonal antibodies were raised in goats against bovine caseins. The antibodies were recovered from crude antisera by ammonium sulfate precipitation, and partially purified by passing them through a DEAE anion exchange column.

Detection of Low Levels of Cows' Milk in Goats Milk

Both assays were able to quantitatively detect 0.0625 to 100% (v/v) cows' milk adulteration of goats' milk. Indirect ELISA can be applied to raw milk, HTST milk, pasteurized milk and sterilized milk, while sandwich ELISA can be only applied to raw milk. The results of the indirect ELISA assays correlated very well with those of urea SDS-PAGE (polyacrylamide gel electrophoresis). However, indirect ELISA is more sensitive, and capable of providing a quantitative analysis.

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