Development of a Differential Diagnostic Reagent for the Detection of Classical Swine Fever

News source: National Chung Hsing University, Taiwan, ROC.
For further information: Taiwan Jour. Vet. Med. Anim. Husb., 67, 127-134, 1997; Virus Genes, 16, 227-236, 1998., 2001-01-01

Differentiating the Disease from the Vaccine

Classical swine fever (CSF), also named hog cholera, is an important infectious disease of pigs. In order to eradicate this disease, a differential diagnostic reagent to differentiate the natural infection from vaccination needs to be developed. The capsid protein gene of the CSF virus (CSFV) was cloned and expressed in E. coli. The expressed protein was identified as a product of 38.5 kDa. The product is a fusion protein with six histidines at its N-terminal. It can easily be purified by the method of nickle-chelate affinity chromatography.

Use As Antigen

The purified product can be used as an antigen in the ELISA for the detection of antibodies against CSFV. According to preliminary data, this ELISA method was more sensitive and more specific than commercial products available on the market. Also, this product can be used for differential diagnosis when the vaccine used is the subunit vaccine, using envelope proteins as an antigen.

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