Culture of Acacia

Some experiments were carried out to find the optimal cultural conditions for the efficient propagation of hybrid Acacia by tissue culture. The following results were obtained.

• 1) Buds for plantlets were successfully sterilized with 0.1 % HgCl2 solution.
• 2) May and August were the best season for the collection of buds.

Foundation Media

• 3) For the foundation media, Murashige & Skoog (MS) was used. The best combination and concentration of growth regulators for rooting the materials differed according to the hybrid line. For instance, for line No. 10, a combination of IBA 3.0-5.0 mg/L, NAA 1.0 mg/L, and IAA 3.0 mg/L was the best, For line No. 32, a combination of IBA 2.0-4.0 mg/L, NAA 1.0 mg/L, and IAA 2.0-3.0 mg/L was the best.

Growing Media

• 4) Rooted plantlets were transplanted into sand media in a greenhouse 20 days after being sprayed with benlate. Later, they were transplanted again into plastic bags filled with soil, and were kept in a nursery bed outdoors.
• 5) Thus it was found that tissue culture was a practical way of propagating hybrid Acacia.

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