Biochip Diagnosis System for Classical Swine Fever Virus (CSFV)
Bio-chip is one of the major biological techniques in recent years. It can detect different pathogens in a piece of chip and can differentiate the various genotypes in the same species. In 2003, a bio-chip diagnosis system was established by using classical swine fever virus (CSFV) as a model. Nucleotide sequences of classical swine fever virus were collected from the GenBank, and the domestic CSFV field strains were sequenced. These sequence data were analyzed with a computer software by sequence alignment and a pair of universal primers, which could amplify different genotypes of CSFV. Also, a specific probe from the amplification region was designed. The specific probe was synthesized and dot-blotted on the bio-chip.
Able to Differentiate Three Genotypes of CSFV
By the hybridization patterns, the distinct genotypes of CSFV could be differentiated. The results showed that the CSFV amplified by primer CP5.6 could be classified into three genotypes, including the native, invaded, and lapinized hog cholera vaccine virus by hybridization with oligonucleotide probe. No cross reaction was found when the bio-chip was hybridized with bovine diarrhea disease virus which was closely related to the CSFV in antigenicities.
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